cDNA sub-cloning in retroviral expression vectors
CG-00021    Identification and cloning of full length cDNA
CG-00022    Full length cDNA subcloning in retroviral pRNCX vector
CG-00023    Full length cDNA subcloning in retroviral pRPCX vector
CG-00024    Full length cDNA subcloning in Lenti-CMV vector
CG-00030   cDNA mutagenesis
CG-00040    Full length cDNA subcloning in custom vectors
Inquiry
Inquiry
CG-00041    Full length sequencing of the cDNA insert
We offer cDNA expression clone construction service in several retrovirus based vectors listed above.  User supplied vectors are acceptable only in special
circumstance.  Email us at
info@cellogenetics.com for inquiries.  These viral vectors offer highly efficient DNA transfer into proliferating or resting cells and
high level expression of the cDNA insert.  If you already have the cDNA and would like to use one of the listed vectors, please send your clone in whatever form
along with desired requirement, we will subclone it and send you the completed construct as glycerol stock.  

A large number of collections of either partial or full length cDNA clones are now housed by various commercial vendors.  Most of these clones are identified
directly from cDNA libraries by sequencing and therefore are unprocessed.  If you don't have the cDNA clone, or if you would like to introduce functional
mutations, we can identify the most suitable clones from those open collections and use them as starting material for constructing your desired expression
cloens.  

The typical turn around time for completing the expression clone construction is 3 weeks from the date when the order and starting cDNA clone are received.

The plasmid map and technical information are available following the above links.  If desired, we also offer to sequence the entire cDNA insert.


cellogenetics, inc