pLenti-hU6BX vector, please submit your gene name, accession #, target and
loop sequences here.
conditions listed on the ordering page. Submitting an order form is construed as
you have carefully read and agree with such terms and conditions.
Purchase of the shRNA vector Construction service amounts to entering a service
agreement under which Cellogenetics is contractually obligated to:
1. synthesize the corresponding DNA oligoes for and generate the ordered shRNA
expression constructs.
2. confirm the cloned shRNA coding sequence by DNA sequencing.
3. make glycerol stocks of bacterial clones containing the ordered shRNA
expression constructs and prepare technical spec sheet.
4. ship the ordered shRNA expression constructs as glycerol stocks along with all
pertinent technical information within 15 business days from the date when the
order was placed.
vector designed to express double stranded small hairpin RNA
(shRNA) from the human U6 snRNA promoter. There are four base
changes between the +1 site (G) and the TATA box in the human U6
promoter inserted in pLenti-hU6BX. Mutagenesis studies reported in
the literature indicate that these four bases are not conserved and
that the alteration shown here would not affect transcription
efficiency.
All pLenti-hU6BX derived plasmids should be propagated in suitable
host strains such as DH5a, or HB101, under selection of resistance to
ampicillin (50 ug/ml). E.coli replication origin: pUC; copy number:
high.
Infectious viral particles should be obtained from 293T cells by
co-transfection with helper plasmids: delta8.9 (gag/pol) and vsv-g
(pseudotyping plasmid), with the Virapower packaging mix
(K4970-00, InVitrogen).
